in vitro Bioakkumulation

© Stefan Stolte. Click to enlarge the figure.

Bioaccumulation is of highest concern for environmental risk assessment of chemicals, since it is known to cause far‐reaching hazards to wildlife and human health. Generally, the experimental measurement of bioaccumulation is time‐consuming, expensive, and due to ethical concerns regarding animal welfare not feasible for large sets of chemicals.

Thus, prediction models ‐ mainly based on easily determinable physicochemical properties such as the octanol‐water partition coefficient ‐ are used for the risk assessment.

The existing prediction models are applicable to hydrophobic and polar organic chemicals; however, they often give inappropriate and inaccurate results for ionogenic compounds and permanently charged organic chemicals. This is due to the fact that classical bioaccumulation models neither sufficiently consider ion–macromolecule interactions nor interactions of cations and anions in solution ‐ both strongly influencing the transport, uptake and bioavailability of ions.

The main aim of the proposed project is to understand and predict the interactions of organic ions, and ion pairs in particular, with biological systems and their consequences in terms of bioaccumulation. Therefore a mechanistic approach applying several in vitro tests such membrane and storage lipid partitioning and protein binding is employed to achieve comprehensive data sets of charged and ionogenic compounds.  

Contact:

Dr. Joanna Mazskowska

Dr. Stefan Stolte