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Aktuell: Masterarbeit für ein chemisch analytisches Thema (LC-MS) zu vergeben

Identifizierung von Amphidinolen in Kulturen der fischgiftigen Algenart Amphidinium und Entwicklung einer LC-MS/MS Methode zu deren quantitativem Nachweis.

Fischgiftige Algenblüten sind seit langem bekannt, bekommen mit steigender Nutzung mariner Ressourcen und Etablierung von Fisch-Aquakulturen zunehmend an Bedeutung, weil Fische in Käfigen gehalten werden und deswegen natürlichen Bedrohungen nicht ausweichen können. Obwohl man viele fischgiftige (ichthyotoxische) Algenarten kennt, ist sehr wenig über die fischgiftigen Substanzen bekannt. Zwei Ausnahmen stellen die Gattungen Karlodinium und Amphidinium, die jeweils sehr ungewöhnliche und große Moleküle im Massenbereich um 1300 Da produzieren. Während die Karlotoxine schon relativ gut untersucht sind, ist wesentlich weniger über Amphidinole bekannt.

Ziel der Arbeit ist in Biomasse der Art Amphidinium klebsii die vorkommenden Amphidinole zu identifizieren und massenspektrometrisch zu charakterisieren. Dazu soll Triple Quadrupol Tandem Massenspektrometrie gekoppelt mit Flüssigchromatographie (LC-MS/MS) eingesetzt um über Full Scan, Precursor Ionen- und Produkt Ionen Experimente die Amphidinole zu identifizieren und dann zu charakterisieren. Auf Grundlage dieser Ergebnisse soll dann eine Quantifizierungsmethode für die vorliegenden Amphidinole im Selected Reaction Monitoring (SRM) Modus entwickelt werden.

Bei Interesse bitte bei Prof. Harder melden.

New collaborative EU ERA NET Project

Protist Metabolome Screening - PROMiSE [PDF] (271 KB)

Marine eukaryotic protists offer a huge but currently underexploited reservoir of metabolic pathways with biotechnological potential. Given their unique adaptations through symbiosis, endosymbiosis and organelle acquisition, the ecofunctionalities of protists present a hitherto untapped source to discover novel metabolic pathways and bioactivities whilst bearing a high chance of discovering different  activities compared to those identified in marine sources, such as bacteria.

The scientific approach and rationale sets PROMiSE apart from many previous scientific initiatives exploiting the biotechnological potential of marine bacteria. The PROMiSE experimental workflow enables this by employing a comprehensive set of Omics methods. This approach spans the encoded metabolic potential to identify biosynthetic gene clusters which in turn guide the targeted metabolite profiling, merged with discovery-based metabolomics. The goal is to target identified candidate compound classes and their pathway-related metabolites and conjugations dereplicated from the Omics information. By linking these methods back to the source cell through single cell Omics methods, PROMiSE offers a unique way to recognize functional gene clusters and to understand how metabolism is partitioned across ecosystems.

This is important to unravel how the identified pathways work in nature, and by extension, how they can be expressed and utilized for technological adaptations relevant to a human health and biotechnology market. The vertically integrated extraction and analyses procedure within PROMiSE are supported by a comprehensive array of cutting-edge in vitro and in vivo bioassays for reliably assessing biological activities by High-Content profiling and antibacterial screening. Analytical chemistry, including high resolution mass spectroscopy and nuclear magnetic resonance spectroscopy approaches, will be used to elucidate compounds found in the bioactive fractions, which will tie back the molecular data to identify relevant enzymes, pathways, and compounds.

Tailored biobased ingredients for a competitive bioeconomy

In this new BMBF funded project we will in collaboration with the Alfred Wegener Institute and 2 SME develop a formulated dietary supplement made from marine algae. Specifically, we aim to capitalize on a specific but biotechnologically neglected property of algal food, i.e. their neuroprotective effect on brain cells.

Hosting a Visiting Scientist

Prof. Michiya Kamio from Tokyo University of Marine Science and Technology will spend his sabbatical in our group for one year to work on the structural elucidation of marine signalling compounds.

Aktualisiert von: Tilmann Harder