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Masterarbeit: Identification of growth conditions on cathode of hyperthermophilic archaea

IGCC Master

Starting from October 2020, the Research Group Environmental Process Engineering at the University of Bremen (Germany) seeks candidates for a 6-months Master Thesis in the field of electromicrobiology.
The project of this Master Thesis takes place in a French-German collaboration between the MIO - Mediterranean Institute of Oceanography (Marseille, France) and the Research Group Environmental Process Engineering (Bremen, Germany). It aims to identify the best condition for the growth of biofilm on cathode of pure culture of hyperthermophile microorganisms. Representants of 5 different phylogenetic groups of Archaea and Bacteria have been previously observed in electroactive communities growing on cathode of Microbial Electrosynthesis System inoculated with deep-sea hydrothermal vent samples and operated at 80°C. These microorganisms were affiliated to Geoglobus, Ferroglobus, Archaeoglobus, Aquifex and Thermococcus. In order to study the electroactive ability of members of these genera, 6 pure cultures will be separately tested in 6-electrode reactors in different conditions to obtain significant biofilm on electrode. The conditions to optimized will be the pH, the potential of the cathode, the electron acceptor and the material or surface modification of the electrode. The biofilm will be analyzed by fluorescence microscopy, qPCR, HPLC, SEM and electrochemical technics.

Supervisors:

Prof. Dr.-Ing habil. Sven Kerzenmacher – kerzenmacher@uni-bremen.de
Dr. Guillaume Pillot – pillot@uvt.uni-bremen.de
www.uvt.uni-bremen.de