MRT-Biofilm

The aim of this project is to further develop MRI as an in vivo/in situ technique for investigating the spatial distribution of biomass density, mass transfer properties and substrate (O₂ and H₂) and pH gradients in hydrogenotrophic and/or aerophilic biofilms. Thus, their influence on reaction rates and biomass growth will be determined. For this purpose, a biofilm of C. necator will be experimentally examined in an MRI scanner during its growth. Using different MR techniques, the morphology as well as the spatially resolved pH, O₂ and H₂ concentrations within the biofilm will be characterized and locally resolved effective diffusion coefficients within the biofilm will be determined. Furthermore, we will expose the biofilms of C. necator to quorum sensing molecules and use our MRI method to dynamically investigate their effects on architecture and productivity as a function of local pH and oxygen conditions.