The purification of proteins is a fundamental step prior to almost every experiment in our lab. A major part of the research of biologists in our lab is the identification and sequencing of proteins from nacre of Haliotis laevigata. From the protein mixture extractable from nacre different proteins have to be isolated for further studies.
Currently different chromatography systems are used for this task. Our lab is equipped with a high pressure liquid chromatography system, an ion exchange chromatography system and gel chromatography columns for protein purification and separation.
An UV-VIS spectrophotometer and a luminescence spectromenter can be used for further analysis of the proteins. Additionally microcrystal binding experiments are conducted to reveal the function of proteins from nacre.
Author: Malte Launspach