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Original publications

Ayala Mariscal SM, Pigazzini ML, Richter Y, Özel M, Grothaus IL, Protze J, Ziege K, Kulke M, ElBediwi M, Vermaas JV, Colombi Ciacchi L, Köppen S, Liu F, Kirstein J

Identification of a HTT-specific binding motif in DNAJB1 essential for suppression and disaggregation of HTT.

Nat Commun. 2022 Aug 10;13(1):4692



Venugopalan V, Rehders M, Weber J, Rodermund L, Al-Hashimi A, Bargmann T, Golchert J, Reinecke V, Homuth G, Völker U, Verrey F, Kirstein J, Heuer H, Schweizer U, Braun D, Wirth EK, Brix1 K

Lack of L-type amino acid transporter 2 in murine thyroid tissue induces autophagy.

Journal of Molecular Endocrinology, in press

Pras A, Houben B, Aprile FA, Seinstra R, Gallardo R, Janssen L, Hogewerf W, Gallrein C, Vleeschouwer MD, Mata-Cabana A, Koopman M, Stroo E, Vries MD, Edwards SL, Kirstein J,  Vendruscolo M, Falsone SF, Rousseau F, Schymkowitz J, Nollen EAA 

The cellular modifier MOAG-4/SERF drives amyloid formation through charge complementation. 

EMBO J, accepted,


Pigazzini ML, Lawrenz M, Margineanu A, Kaminski Schierle GS, Kirstein J 

An expanded polyproline domain maintains mutant huntingtin soluble in vivo and during aging. 

Frontiers in Molecular Neuroscience, accepted, doi 10.3389/fnmol.2021.721749

Venugopalan V, Al-Hashimi A, Rehders M,  Golchert J, Reinecke V, Homuth G, Völker U, Manirajah M, Touzani A, Weber J, Bogyo MS, Verrey F, Wirth EK, Schweizer U, Heuer H, Kirstein J, Brix K

The thyroid hormone transporter Mct8 restricts cathepsin-mediated thyroglobulin processing in male mice through thyroid auto-regulatory mechanisms that encompass thyrotoxicity-induced autophagy

International Journal of Molecular Sciences, 2021 Jan 5;22(1):462

Gallrein C, Iburg M, Michelberger T, Koçak A, Puchkov D, Liu F, Ayala Mariscal SM, Nayak T, Kaminski Schierle GS, Kirstein J 

Novel Amyloid-beta pathology C. elegans model reveals distinct neurons as seeds of pathogenicity

Progress in Neurobiology, Sep 11:101907

Haenig C, Atias N,  Taylor AK, Mazza A, Schaefer MH, Russ J, Riechers SP, Jain S, Coughlin M, Fontaine JF, Freibaum BD, Brusendorf L, Zenkner M, Stroedicke M, Schnoegl S, Arnsburg K, Boeddrich A, Pigazzini ML, Heutink P, Taylor JP, Kirstein J, Andrade-Navarro MA, Sharan R, Wanker EE 

Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains

Cell Reports, 2020 Aug 18;32(7):108050. doi: 10.1016/j.celrep.2020.108050.

Pigazzini ML &, Kirstein J 

In vivo quantification of protein turnover in aging C. elegans using the photoconvertable Dendra2
J. Vis Exp. 2020 Jun 13;(160). doi: 10.3791/61196.

Iburg M, Puchkov D, Rosas-Brugada IU, Bergemann L, Rieprecht U, Kirstein J

The non-canonical small heat shock protein HSP-17 from C. elegans is a selective protein aggregase.

J Biol Chem 2020 doi: 10.1074/jbc.RA119.011185

Pigazzini ML, Gallrein C, Iburg M, Kaminski Schierle G, Kirstein J

 Characterisation of amyloid structures in aging C. elegans using fluorescence lifetime imaging. 
J. Vis Exp. 2020 Mar 27;(157). doi: 10.3791/61004.


Claes F, Rudyak S, Laird AS, Louros N, Beerten J, Debulpaep M, Michiels E, van der Kant R, Van Durme J, De Baets G, Houben B, Ramakers M, Yuan K, Gwee SSL, Hernandez S, Broersen K, Oliveberg M, Moahamed B, Kirstein J, Robberecht W, Rousseau F, Schymkowitz J 

Exposure of a cryptic Hsp70 binding site determines the cytotoxicity of the ALS-associated SOD1-mutant A4V

Protein Engineering, Design and Selection, 2019 Dec 31;32(10):443-457. doi: 10.1093/protein/gzaa008.

Sandhof CA, Hoppe SO, Druffel-Augustin S, Gallrein C, Kirstein J, Voisine C, Nussbaum-Krammer C 

Reducing INS-IGF1 Signaling Protects Against Non-Cell Autonomous Vesicle Rupture Caused by SNCA Spreading.

Autophagy, 2019 Jul 29:1-22

Feleciano DR, Juenemann K, Iburg M, Bras I, Holmberg CI, Kirstein J 

Crosstalk between chaperone-mediated protein disaggregation and proteolytic pathways in aging and disease.

Frontiers in Aging Neuroscience, 2019 Jan 29;11:9

Kreis P*, Gallrein C, Rojas-Puente E, Mack TGA, Kroon C, Dinkel V, Willmes C, Murk K, tom-Dieck S, Schuman EM, Kirstein J*, Eickholt BJ* 

ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance and lifespan.

Nature Communications, 2019 Jan 30;10(1):486 (* co-corresponding authors)


AstA, Buntru A, SchindlerF, HasenkopfR, SchulzA, BrusendorfL, KlockmeierK, GrelleG, McMahonB, NiederlechnerH, JansenI, DiezLM, BoeddrichA, FranklinSA, BaldoB, SchnoeglS, KunzS, PurfürstB, KampingaHH, MortonAJ, PetersénA, KirsteinJ, BatesGP, Wanker EE

Mutant HTT seeding activity: a marker of disease progression and neurotoxicity in models of Huntington’s disease.

Molecular Cell, 2018 Sep 6;71(5):675-688.e6

Juenemann K, Jansen AHP, van Riel L, Merkx R, Mulder MPC, An H, Statsyuk A, Kirstein J, Ovaa H, Reits EA 

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.

Scientific Reports, 2018 23;8(1):1405

Scior A, Arnsburg K, Iburg M, Juenemann K, Mlody B, Pigazzini ML, Buntru A, Puchkov D, Wanker EE, Prigione A, Priller J & Kirstein J 

Complete suppression of Htt aggregation and disaggregation of Htt fibrils by a specific trimeric chaperone complex.

The EMBO Journal, 2018; 37(2)282-299

KirsteinJ, ArnsburgK, SciorA, SzlachcicA, GuilbrideDL, MorimotoRI, BukauB, Nillegoda NB 

In vivo properties of the disaggregase function of J-proteins and Hsc70 in C. elegans stress and aging.

Aging Cell, 2017 16(6):1414-1424

Lechler MC, Crawford ED, Groh N, Widmaier K, Jung R, Kirstein J, Trinidad JC, Burlingame AL, David DC

Reduced Insulin/IGF-1 Signaling Restores the Dynamic Properties of Key Stress Granule Proteins during Aging.

Cell Reports, 2017; 18(2):454-467

Nillegoda NB, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, Aebersold R, Guilbride DL, Wade RC, Morimoto RI, Mayer MP, Bukau B 

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation.

Nature 2015; 524(7564)

Kirstein J*, Morito D, Kakihana T, Sugihara M, Minnen A, Hipp MS, Nussbaum-Krammer C, Hartl FU, Nagata K, Morimoto RI 

Proteotoxic stress and ageing triggers the loss of redox homeostasis across cellular compartments.

The EMBO Journal 2015; 34(18). (*co-corresponding author)

Kirstein-Miles J, Scior A, Deuerling E, Morimoto RI 

The nascent polypeptide-associated complex is a key regulator of proteostasis.

The EMBO Journal 2013; 32(10).

Rampelt H, Kirstein-Miles J, Nillegoda NB, Chi K, Scholz SR, Morimoto RI, Bukau B 

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation.

The EMBO Journal 2012; 31(21).

Lowth BR, Kirstein-Miles J, Saiyed T, Brötz-Oesterhelt H, Morimoto RI, Truscott KN, Dougan DA 

Substrate recognition and processing by a Walker B mutant of the human mitochondrial AAA+ protein CLPX.

Journal of Structural Biology 2012; 179(2).

Wolf D, Kalamorz F, Wecke T, Juszczak A, Mäder U, Homuth G, Jordan S, Kirstein J, Hoppert M, Voigt B, Hecker M, Mascher T 

In-Depth Profiling of the LiaR Response of Bacillus subtilis.

Journal of Bacteriology 2010; 192(18).





Kirstein J, Hoffmann A, Lilie H, Schmidt R, Rübsamen-Waigmann H, Brötz-Oesterhelt H, Mogk A, Turgay K 

The antibiotic ADEP reprogrammes CIP, switching it from a regulated to an uncontrolled protease.

EMBO Molecular Medicine 2009; 1(1).

Kirstein J, Strahl H, Molière N, Hamoen LW, Turgay K 

Localization of general and regulatory proteolysis in Bacillus subtilis cells.

Molecular Microbiology 2008; 70(3).

Haslberger T, Zdanowicz A, Brand I, Kirstein J, Turgay K, Mogk A, Bukau B 

Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments.

Nature Struct. Mol. Biol. 2008 15, 641-650.

Erbse AH, Wagner JN, Truscott KN, Spall SK, Kirstein J, Zeth K, Turgay K, Mogk A, Bukau B, Dougan DA 

Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding.

FEBS J. 2008 275, 1400-1410.

Kirstein J, Dougan DA, Gerth U, Hecker M, Turgay K 

The tyrosine kinase McsB is a regulated adaptor protein for ClpCP.

The EMBO Journal 2007; 26(8).

Kirstein J, Schlothauer T, Dougan DA, Lilie H, Tischendorf G, Mogk A, Bukau B, Turgay K 

Adaptor protein controlled oligomerization activates the AAA+ protein ClpC.

The EMBO Journal 2006; 25(7).

Andersson FI, Blakytny R, Kirstein J, Turgay K, Bukau B, Mogk A, Clarke AK 

Cyanobacterial ClpC/HSP100 protein displays intrinsic chaperone activity.

Journal of Biological Chemistry 2006; 281(9).

Kirstein J*, Zühlke D, Gerth U, Turgay K, Hecker M

A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis.

The EMBO Journal 2005; 24(19). (* corresponding author)

Gerth U, Kirstein J, Mostertz J, Waldminghaus T, Miethke M, Kock H, Hecker M 

Fine-Tuning in Regulation of Clp Protein Content in Bacillus subtilis.

Journal of Bacteriology 2004; 186(1).

Rollenhagen C, Antelmann H, Kirstein J, Delumeau O, Hecker M, Yudkin MD 

Binding of A and B to Core RNA Polymerase after Environmental Stress in Bacillus subtilis.

Journal of Bacteriology 2003; 185(1).



Ayala Mariscal SM, Kirstein J

J-domain proteins interaction with neurodegenerative disease-related proteins.

Experimental Cell Research, 2021, in press

Kampinga HH, Andreasson C, Barducci A, Cheetham ME, Cyr D, Emanuelsson C, Genevaux P, Gestwicki JP,  Goloubinoff P, Huerta-Cepas J, Kirstein J, Liberek K, Mayer MP, Nagata K, Nillegoda NB, Pulido P, Ramos C, De los Rios P, Rospert S,  Rosenzweig R, Sahi C, Taipale M, Tomiczek B, Ushioda R, Young JC, Zimmermann R, Zylicz A, Zylicz M, Craig EA, Marszalek J

Function, evolution, and structure of J-domain proteins.

Cell Stress and Chaperones, 2019 Jan;24(1):7-15. 

Scior A, Juenemann K, Kirstein J: Cellular strategies to cope with protein aggregation. Essays in Biochemistry, 2016; 60(2):153-161
Feleciano DR, Arnsburg K, Kirstein J: Interplay between redox and protein homeostasis. Worm 2016; 5(2).
Feleciano DR, Kirstein J: Collapse of redox homeostasis during aging and stress. Molecular & Cellular Oncology 2015; 3(2).
Arnsburg K, Kirstein-Miles J: Interrelation Between Protein Synthesis, Proteostasis and Life Span. Current Genomics 2014; 15(1).
Kirstein-Miles J, Morimoto RI: Ribosome-associated chaperones act as proteostasis sentinels. Cell cycle 2013; 12(15).
Kirstein-Miles J, Morimoto RI: Caenorhabditis Elegans as a Model System to Study Intercompartmental Proteostasis: Interrelation of Mitochondrial Function, Longevity, and Neurodegenerative Diseases. Developmental Dynamics 2010; 239(5)
Kirstein-Miles J, Morimoto RI: Peptides Signal Mitochondrial Stress. Cell metabolism 03/2010; 11(3).
Kirstein J, Molière N, Dougan DA, Turgay K: Adapting the machine: Adaptor proteins for Hsp100/Clp and AAA+ proteases. Nature Reviews Microbiology 09/2009; 7(8).
Kirstein J, Turgay K: A New Tyrosine Phosphorylation Mechanism Involved in Signal Transduction in Bacillus subtilis. Journal of Molecular Microbiology and Biotechnology 02/2005; 9(3-4).